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Determination of substrate specificity of Re-citrate synthase
| 2-Oxoacid substrate + acetyl-CoA | Corresponding enzyme | MnCl2 (0.2 mM) | Sp act (U/mg) |
|---|---|---|---|
| Pyruvate | Citramalate synthase | +/− | NDa |
| 2-Oxoglutarate | Homocitrate synthase | +/− | ND |
| 2-Oxo-3-methylbutanoate | Isopropylmalate synthase | +/− | ND |
| Oxaloacetate | Re-Citrate synthase | + | 1.2 |
| Oxaloacetate | Re-Citrate synthase | − | 0.72 |
| Oxaloacetate + propionyl-CoAb | Methylcitrate synthase | + | ND |
ND, not detected.
Acetyl-CoA was replaced by propionyl-CoA.
