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Effects of the growth medium on unregulated replication in topA and rnhA mutants. Rifampin run-out experiments for flow cytometry analyses were performed as described in Materials and Methods. (a) RFM443 (wild-type), RFM445 [gyrB(Ts)], and RFM475 [gyrB(Ts) topA] cells grown in LB or M9 glucose medium; (b) RFM430 (wild-type) and PH379 (rnhA) cells grown in LB or M9 glucose medium.
