Table 1.
Bacterial strains and plasmids used in this study and their relevant characteristics
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli DH5α | F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rK− mK+) deoR thi-1 supE44 gyrA96 relA1 λ− | Invitrogen |
| Erwinia amylovora | ||
| Ea1189 | Wild type | 37 |
| Ea1189Δhfq | hfq deletion mutant, Cmr | This study |
| Ea1189ΔhrpL | hrpL deletion mutant, Cmr | 10 |
| Ea1189Δams | Deletion of 12-gene ams operon, Cmr | 37 |
| Ea1189ΔT3SS | Deletion of 24-gene T3SS pathogenicity island, Kmr | 37 |
| Ea1189ΔryhA | ryhA sRNA deletion mutant, Cmr | This study |
| Ea1189ΔrprA | rprA sRNA deletion mutant, Cmr | This study |
| Ea1189Δspf | spf sRNA deletion mutant, Cmr | This study |
| Ea1189ΔmicA | micA sRNA deletion mutant, Cmr | This study |
| Ea1189ΔomrAB | omrAB sRNA deletion mutant, Cmr | This study |
| Ea1189ΔryhB | ryhB sRNA deletion mutant, Cmr | This study |
| Ea1189ΔsroB | sroB sRNA deletion mutant, Cmr | This study |
| Ea1189ΔryeA | ryeA sRNA deletion mutant, Cmr | This study |
| Ea1189ΔglmZ | glmZ sRNA deletion mutant, Cmr | This study |
| Plasmids | ||
| pKD4 | Apr Kmr, mutagenesis cassette template | 38 |
| pKD46 | Apr, expresses bacteriophage λ red recombinase | 38 |
| pML123 | RSF1010-derived expression and lac fusion broad-host-range vector, Gmr | 39 |
| pMLhfq | 530-bp fragment containing hfq with its native promoter cloned at XbaI/SacI in pML123, Gmr | This study |
| pLRT201 | pMJH20 expressing DspE(1-737)-CyaA | 40 |
| pLRT8 | pMJH20 expressing DspE(1-15)-CyaA | 40 |
a
Cmr, Kmr, Gmr, and Apr, chloramphenicol, kanamycin, gentamicin, and ampicillin resistance, respectively.