Fig 2.

Rv3852 binds to DNA and changes the nucleoid morphology. (A) The DNA binding property of Rv3852 was analyzed by EMSA using 30 bp double-stranded DNA (dsDNA). (B) Fluorescent micrographs of the DAPI-stained nucleoid of M. smegmatis cells. (Left) Condensed nucleoid in control cells. (Middle) Decompaction of nucleoid in Rv3852-expressing cells. (Right) Compact nucleoid in Rv3852ΔCTD-expressing cells. The cells shown are representative of ∼85% of the population. Bars, 1 μM. DIC, differential interference contrast.