Table 3.
Effects of variable mutations, including the substitution mutations of the respective adenines at nucleotide +1 of PkinA and PkinB with guanines, on sporulation efficiency
| Strain | Sporulation percentage (%)a |
|
|---|---|---|
| Decoyinine induced, S6 medium, T10 | Nutrient medium (NSMP), T20 | |
| Wild-type 168 | 19 | 71 |
| PS37t (ΔcodY) | 36 | 29 |
| FU1106 (ΔabrB) | 0.40 | 34 |
| FU1121 (Δspo0A) | <5 × 10−6 | <5 × 10−6 |
| ASK201 (Δspo0H) | <5 × 10−6 | <5 × 10−6 |
| FU1095 (ΔkinA) | 0.26 | 7.9 |
| FU1063 (ΔkinA ΔcodY) | 7.3 | 8.5 |
| FU1096 (ΔkinB) | 0.67 | 22 |
| FU1064 (ΔkinB ΔcodY) | 1.5 | 12 |
| FU1098 (ΔkinA ΔkinB) | <5 × 10−6 | <5 × 10−6 |
| FU1102 [kinA (A + 1G)] | 3.5 | 36 |
| FU1103 [kinA (A + 1G) ΔkinB] | 6.2 × 10−4 | 0.055 |
| FU1113 [kinB (A + 1G)] | 1.7 | 25 |
| FU1114 [ΔkinA kinB (A + 1G)] | 2.1 × 10−5 | 0.0019 |
| FU1155 [kinA(A + 1G) kinB (A + 1G)] | 0.0011 | 2.3 |
| FU1156 [kinA (A + 1C)] | 4.2 | 27 |
| FU1170 [kinA (A + 1C) ΔkinB] | 7.6 × 10−5 | 0.0052 |
a
The sporulation experiments were repeated at least three times. Representative values are presented. The standard deviations were less than 15% of the values shown.