Effects of Veg inactivation and overproduction on the architecture of colonies and pellicles. Wild-type (WT) (168 BFA or NCIB3610), Δveg (LY011 [168 BFA harboring Δveg] or LY048 [NCIB3610 harboring Δveg]), pO-veg (LY016 [168 BFA harboring pO-veg] or LY058 [NCIB3610 harboring pO-veg]) and pO-MCS (LY015 [168 BFA harboring pO-MCS] or LY057 [NCIB3610 harboring pO-MCS]) strains were grown in the indicated media for 72 h (168 background strains) or 48 h (NCIB3610 background strains) at 30°C. The LY015, LY016, LY057, and LY058 strains were grown in the presence of 1 mM IPTG. Scale bar, 0.5 cm.