Fig 3.
Overproduction and deletion of Veg induces and suppresses transcription of the tasA operon. (A) Expression of Peps-lacZ and that of PtapA-lacZ were compared after cultivation for 72 h (168 BFA background strains) or 24 h (NCIB3610 background strains) at 30°C on solid MSgg medium containing X-Gal and 1 mM IPTG, for strains with a mutation(s) and/or plasmid, indicated at the top of each picture. All strains contain an epsH mutation to abolish cell aggregation, and strains in the top and bottom panels have insertion of Peps-lacZ and PtapA-lacZ at the amyE loci, respectively. Scale bar, 0.2 cm. (B) Growth and expression of PtapA-lacZ in wild-type (LY032, diamonds), Δveg (LY041, circles), pO-veg (LY039, squares) and pO-MCS (LY038, triangles) strains in LB liquid medium, indicated with open and closed symbols, respectively, were measured. The LY036, LY037, LY038, and LY039 strains were grown in the presence of 1 mM IPTG.