Table 1.
Strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| L. pneumophila strains | ||
| Philadelphia-1 | Spontaneous Strr mutant of Philadelphia-1 | 40 |
| 130b (AA100) | ATCC BAA-74 | |
| AA100ΔdsbA1 | Deletion of dsbA1 | 10 |
| E. coli strains | ||
| DH5α | F− endA1 hsdR17 (rK− mK−) supE44 thi-1 recA1 gyrA (Nalr) relA1 (lacIZYA argF)U169 deoR (80dlac(lacZ)M15) | Clontech |
| BL21(DE3) | CodonPlus RIL B F− ompT hsdS(rB− mB−) dcm Tetr gal (DE3) endA hte argU ileY leuW Camr | Stratagene |
| JCB570 | ER1821 | J. C. Bardwell |
| JCB571 | dsbA | J. C. Bardwell |
| RGP209 | ER1821 dsbC | 20 |
| RGP663 | ER1821 + pPDI detector plasmid | 20 |
| RGP665 | RGP209 + pPDI detector plasmid | 20 |
| Plasmids | ||
| pPDI detector | pBR322 Bla S81C, T108C | 20 |
| pET15b | Expression vector, His tag | Novagen |
| pBC | pBSK with chloramphenicol cassette | Stratagene |
| pMMB206 | Derivative of pRSF1010; Ptac promoter and IPTG-inducible lacIQ system; Cmr | 42 |
| pET15bdsbA1 | dsbA1 His tag | 10 |
| pET15bdsbA2 | dsbA2 His tag | 10 |
| pET15bdsbA2N | dsbA2 His minus dimerization domain | This study |
| pET15bdsbA2LDN | dsbA2 His minus leader and dimerization sequences | This study |
| pBCdsbA1 | dsbA1 in pBC cloning vector | This study |
| pBCdsbA2 | dsbA2 in pBC cloning vector | This study |
| pBCdsbA2N | dsbA2 minus dimerization domain in pBC | This study |
| pBCdsbA2LDN | dsbA2 minus leader and dimerization sequences | This study |