Fig 7.

VCA0685-7-dependent survival under phosphate limitation. Shown is the CI, which reflects the survival ratio of the WT and ΔVCA0685-7 mutant strains. The CI is expressed as a ratio of ΔVCA0685-7 CFU to WT CFU at 3 to 24 h normalized for the input ratio at time zero. Closed circles show CI values derived from cultures incubated in low-phosphate M9-Tris, and square symbols indicate CI values of cultures incubated in standard phosphate M9-Tris medium. Prior to M9-Tris incubation, cultures were grown in M9 supplemented with glycerol and glucose-6P (for details, see the text). Shown are the means of at least four independent measurements. Error bars indicate standard deviations.