Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| E. coli strains | ||
| DH5αλpir | F− Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 λ::pir | 28 |
| SM10λpir | thi recA thr leu tonA lacY supE RP4-2-Tc::Mu λ::pir | 29 |
| V. cholerae strains | ||
| E7946 | O1 El Tor, Ogawa; Smr (WT) | 30 |
| ΔVCA0685-7 | Deletion of VCA0685, VCA0686, and VCA0687 in E7946; Smr | This work |
| ΔuhpA | Deletion of VCA0682 in E7946; Smr | This work |
| VCA0687::phoA | Insertion of pGPphoA downstream of VCA0687 in E7946; Smr Apr | This work |
| ΔuhpA VCA0687::phoA | Insertion of pGPphoA downstream of VCA0687 in ΔuhpA; Smr Apr | This work |
| Plasmids | ||
| pGP704 | ori6K mobRP4, Apr | 29 |
| pCVD442 | ori6K mobRP4 sacB, Apr | 30 |
| pTRC99A | pBR322 origin, Apr | 31 |
| pGPphoA | pGP704 with promoterless phoA of SM10λpir, Apr | 32 |
| pGPphoA-VCA0687 | pGPphoA with VCA0687 gene fragment, Apr | This work |
| pCVDΔVCA0685-7 | pCVD442 with upstream fragment of VCA0685 and downstream fragment of VCA0687, Apr | This work |
| pCVDΔuhpA | pCVD442 with up- and downstream fragments of VC0682, Apr | This work |
| pTRCuhpA | pTRC99A with reading frame VCA0682, Apr | This work |
| pTRC0685-7 | pTRC99A with reading frames VCA0685 to VCA0687, Apr | This work |