Table 2.
Oligonucleotides used in this study
| Primer | Sequence (5′ to 3′)a |
|---|---|
| VCA0685-NcoI-2 | TTTCCATGGGTTTTGCATTAACAATCATTGGGTT |
| VCA0685-SacI-1 | TTTGAGCTCATTCACGTAGCGAACGGGGCT |
| VCA0687-XbaI-4 | TTTTCTAGACTCAATTTTGAAGACTATGTGCTC |
| VCA0687-NcoI-3 | TTTCCATGGCAGAATAGTGATAATACCCTGAG |
| VCA0687-KpnI-phoA-3′ | TTTGGTACCTATTATCACTATTCTGCGTAGGG |
| VCA0687-SacI-phoA-5′ | TATGAGCTCTTAGATGCCAACTTGCGCCG |
| UhpA-XbaI-1 | AAATCTAGAGCATCTCCACGTTCAGCCCTAG |
| UhpA-KpnI-2 | AAAGGTACCATGATGTTTATCACCTAGCAAAATC |
| UhpA-KpnI-3 | AAAGGTACCATGTGATCCGCTTTGCTTTGCAG |
| UhpA-SacI-4 | ATTGAGCTCAACGGATTTGCGAATTTGCTCTT |
| UhpA-EcoRI-5′ | TTTGAATTCTGATTTTGCTAGGTGATAAACATCA |
| UhpA-XbaI-3′ | TTTTCTAGAGTTAACCTTCCACGAGCAGTTG |
| VCA0685-EcoRI-5′ | AAAGAATTCAAGGACACAAGGGAGCATCAA |
| VCA0687-XbaI-3′ | TTTTCTAGAGGCTTTACTCAGGGTATTATCAC |
a
Restriction sites are underlined.