Table 1.
Bacterial strains used in this study
| Strain | Relevant propertya | Descriptionb | Reference or source |
|---|---|---|---|
| S. thermophilus strains | |||
| LMD-9 | Wild type | 32 | |
| CB001 | blp::PcomX-luxAB | 6 | |
| LF121 | blp::PcomS-luxAB | 35 | |
| LF123 | blp::PcomR-luxAB | 35 | |
| TIL773 | Δeep | 14 | |
| TIL1179 | pBV5030::P32 | pBV5030::P32 → LMD-9 | This study |
| TIL1199 | ΔamiA1 amiA3::erm | 5 | |
| TIL1202 | ΔamiCDE::spec | 14 | |
| TIL1209 | comS::spec | comS::spec PCR fragment → LMD-9 | This study |
| TIL1307 | pBV5030::P32-comS | pBV5030::P32-comS → LMD-9 | This study |
| TIL1311 | blp::PcomX-luxAB aphA3 | pGICB004a::PcomX → LMD-9 | This study |
| TIL1314 | blp::PcomX-luxAB aphA3 comS::spec | TIL1209 DNA → TIL1311 | This study |
| TIL1329 | pBV5030::P32-comSΔamiCDE::spec | TIL1202 DNA → TIL1307 | This study |
| TIL1349 | blp::PdprA-luxAB aphA3 | pGICB004a::PdprA → LMD-9 | This study |
| TIL1371 | blp::comX-luxAB aphA3 Δeep | TIL1311 DNA → TIL773 | This study |
| TIL1391 | blp::PcomX-luxAB comS::spec | TIL 1209 DNA → CB001 | This study |
| TIL1392 | blp::PcomX-luxAB comS::spec amiA3::erm | TIL1199 DNA → TIL1391 | This study |
| TIL1393 | blp::PcomX-luxAB comS::spec ΔamiA1 amiA3::erm | TIL1199 DNA → TIL1391 | This study |
| TIL1394 | blp::PcomX-luxAB comS::spec ΔamiA1 | pG+host9::updown.amiA1→ TIL1391 | This study |
| TIL1404 | comS::erm | PCR fragment comS::erm → LMD-9 | This study |
| TIL1405 | blp::PcomX-luxAB comS::erm | TIL1404 DNA → CB001 | This study |
| E. coli strain | |||
| TG1 repA+ | TG1 derivative with repA gene integrated into the chromosome | P. Renault, INRA, Jouy-en-Josas, France |
a
erm, aphA3, and spec indicate resistance to erythromycin, kanamycin, and spectinomycin, respectively.
b
Arrows indicate construction by transformation with chromosomal DNA or plasmid.