Table 2.
Plasmids used in this study
| Plasmid | Relevant propertiesa | Reference or source |
|---|---|---|
| pG+host9 | Erm Ts | 33 |
| pG+host9::updown.amiA1 | Erm; derivative of pG+host9 used for amiA1 gene replacement by double-crossover integration | 5 |
| pGICB004 | Erm Ts; allows the integration of transcriptional fusions to the luxAB reporter genes at the blp locus | 14 |
| pGICB004a | Erm; derivative of pGICB004 containing the aphA3 gene downstream from the luxB gene | B. Fleuchot, INRA, Jouy-en-Josas, France, unpublished data |
| pGICB004a::PcomX | Erm; derivative of pGICB004a used to introduce a PcomX-luxAB transcriptional fusion at the blp locus | This study |
| pGICB004a::PdprA | Erm; derivative of pGICB004a used to introduce a PdprA-luxAB transcriptional fusion at the blp locus | This study |
| pBV5030 | Erm; replicative plasmid in E. coli and Gram-positive bacteria | 34 |
| pBV5030::P32 | Erm; derivative of pBV5030 containing the constitutive promoter P32 of Lactococcus lactis | This study |
| pBV5030::P32-ster1357 | Erm; derivative of pBV5030 allowing overexpression of the ster1357 gene of S. thermophilus LMD-9 | 14 |
| pBV5030::P32-comS | Erm; derivative of pBV5030 allowing overexpression of the comS gene of S. thermophilus LMD-9 | This study |
a
Erm, resistance to erythromycin; Ts, the plasmid encodes a thermosensitive RepA protein.