Fig 4.

The ΔEF2638 mutant exhibits a growth defect when grown with aeration. Bacteria were grown in MM9YE supplemented with 0.3% glucose (A and B) or 0.3% glycerol (C and D), and optical density was measured every 30 min at 600 nm. Cultures were grown with aeration (225 rpm, 37°C) (A, C) or statically (B, D). Wild type (OG1), squares; ΔEF2638 (DV87-4) mutant, circles; ΔEF2638 (EF1116-EF1117)2::EF2638-3 (DV106) mutant, triangles. Representative results from at least two independent experiments are shown.