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Binding of GlxR to the ramA promoter region. DNA fragments covering the ramA promoter region with the wild-type (WT) or mutated (MUT) GlxR site were incubated with the purified GlxR at the indicated concentrations (nM) in the presence of 0.5 mM cAMP or in the absence of cAMP (−cAMP) and analyzed by nondenaturing PAGE. Each well contained 10 nM DNA fragment.
