Fig 6.
T. denticola membrane and soluble fractions contain trimeric forms of Msp. (A) Membrane and soluble fractions of T. denticola (MF and SF, respectively) were phase partitioned with TX-114 followed by SDS-PAGE and immunoblot analysis using anti-MspFl antiserum. Molecular mass standards (kDa) are indicated on the right. (B) Membrane and soluble fractions were separated by SDS-PAGE without (−) and with (+) boiling followed by immunoblot analysis with anti-MspFl antiserum. Molecular mass standards (kDa) are indicated on the left. (C) BN-PAGE showing trimer formation by MspFl. Molecular mass standards (kDa) are indicated on the left.