Fig 5.

HPLC analysis of free amino acids present in spent PB media of P. aeruginosa PAO1 and PW5126. Strains were grown in PB at 37°C. At 6 and 24 h postinoculation, free amino acids present in spent media were derivatized and subsequently separated on a Zorbax Eclipse AAA column. Represented chromatograms are shown for an amino acid mixture (A), uninoculated PB (B), PAO1 at 6 h (C), PW5126 at 6 h (D), PAO1 at 24 h (E), and PW5126 at 24 h (F). Glycine concentrations represent mean values of triplicate samples, and standard deviations are shown.