Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Y. pestis strains | ||
| KIM6+ | pCD1-negative, Pgm+ biovar Medievalis strain | 83 |
| KIM6 | Pgm- and pCD1-negative | 84 |
| KIM6+ ΔphoP | In-frame internal deletion of amino acids 25–75 of PhoP | 29 |
| KIM6+ ΔphoP(pLG338) | Empty vector control (Kan, Tet) | This study |
| KIM6+ ΔphoP(pLGphoP) | Complemented phoP mutant (Kan) | This study |
| KIM6+ ΔphoP(pGFP) | GFP expressing (Amp) | This study |
| KIM6+ ΔpmrA | In-frame internal deletion of amino acids 28–113 of PmrA | This study |
| KIM6+ ΔphoP ΔpmrA | pmrA deletion introduced into the phoP mutant | This study |
| KIM6+ ΔpbgP Δugd | Deletion of aminoarabinose synthesis genes (Kan, Amp) | This study |
| KIM6+ Δugd | Deletion of aminoarabinose synthesis gene (Kan) | This study |
| KIM6+ Δugd(pLGugd) | Complemented ugd mutant (Kan, Amp) | This study |
| GB | Wild-type biovar Orientalis strain | 22 |
| GB SAI2.2 | Y. pestis GB phoP mutant | 22 |
| E. coli strains | ||
| S17-1λpir | Host and conjugation donor strain for pCVD442 and derivatives | 85 |
| TOP-10 | pCR 2.1 TOPO plasmid host strain | Invitrogen |
| D31 | Rough LPS mutant (Str) | 86 |
| Plasmids | ||
| pLG338 | Low-copy-no. plasmid vector (Kan, Tet) | 87 |
| pLGphoP | Complementation plasmid; wild-type phoP gene and promoter cloned into pLG338 (Kan) | This study |
| pLG338-30 | Low-copy-no. plasmid vector (Amp) | 88 |
| pLGugd | Complementation plasmid; wild-type ugd gene and promoter cloned into pLG338-30 (Amp) | This study |
| pCR4Blunt-TOPO, pCR-2.1 TOPO | Cloning vectors (Amp) | Invitrogen |
| pGFP | GFP expression plasmid (Amp) | Clontech |
| pCVD442 | Suicide vector for allelic exchange mutagenesis (Amp) | 38 |
| pKOBEG::sacB | λRed and sacB plasmid for mutagenesis (Cam) | 40 |
a
Antibiotic resistance is noted in parentheses (Kan, kanamycin; Tet, tetracycline; Amp, ampicillin; Str, streptomycin; Cam, chloramphenicol). GFP, green fluorescent protein.