Fig 2.

Mass spectrometry analysis of differentially detergent-extracted cell envelopes. (A) Relative detergent extractability of detected proteins, grouped according to the number of predicted TMDs, presence of predicted signal peptides, lack of TMDs (secreted proteins) and lipoboxes, and lack of TMDs (lipoproteins) and PE/PPE motifs. The remaining proteins are considered soluble. Proteins that are <30% extracted are considered putative inner membrane proteins (IMPs), 31 to 89% extracted are undetermined, and >90% extracted are putative mycobacterial outer membrane proteins (MOMPs). The relative abundance of protein groups is indicated. (B) Functional classification of detected proteins in total CE and MOMP groups, as described by the Mycobrowser (27).