Table 1.
Characterization of marker proteins and candidate MOMPsa
| Protein | Orthologue H37Rv | No. of TMD | SP | Predicted molecular mass (kDa) | Observed molecular mass (kDa) | % extraction, endogenousb | % extraction, +HA tagc |
|---|---|---|---|---|---|---|---|
| FtsQ | Rv2151c | 1 | N | 34 | 40 | 24 | 36 |
| OmpATb | Rv0899 | 1 | N | 34 | 40 | 100 | 97 |
| MctB | Rv1698 | 1 | Y | 32 | 35 | 90 | 82 |
| MMAR_0617 | Rv0312d | 1 | N | 62 | 120, 80 | 100 | 75 |
| MMAR_4057 | Rv1972 | 1 | N | 26 | 34, 25 | 98 | 53 |
| MMAR_4366 | Rv1100 | 1 | N | 22 | 30, 24 | 100 | 93 |
| MMAR_5387 | Rv3835 | 1 | N | 48 | 75, 60, more | 100 | 87 |
a
TMD, predicted transmembrane domains (TMHMM); SP, predicted signal peptide (SignalP).
b
Extractability by OBG of endogenous protein quantified by nanoLC-MS/MS.
c
Extractability of HA-tagged protein by OBG as quantified from immunoblot signals (Fig. 3A) using Quantity One.
d
Closest homologue of MMAR_0617, orthologue of Rv0312c, is MMAR_0562.