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. Author manuscript; available in PMC: 2013 Apr 12.
Published in final edited form as: Biochemistry. 2012 Feb 27;51(9):1996–2007. doi: 10.1021/bi201799a

Figure 6.

Figure 6

Concentration dependence of the pre-steady state rate of correct nucleotide incorporation. (A) A preincubated solution containing 2 μM Dpo3 and Template G (9.6 nM) was mixed with increasing concentrations of dCTP (10 μM – 200 μM) for the indicated time points. The data was fit to Equation 3 to determine the single-exponential rate (kobs). (B) kobs values were plotted as a function of dCTP concentration and fit with Equation 4 to yield kp (0.045 ± 0.008 s−1) and Kd (61 ± 26 μM).