Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Concentration dependence of the pre-steady state rate of incorrect nucleotide incorporation. (A) A preincubated solution containing 2 μM Dpo3 and Template T (9.6 nM) was mixed with increasing concentrations of dTTP (250 μM – 4 mM) for the indicated time points. The data was fit to Equation 3 to determine the single exponential rate (kobs). (B) kobs values were plotted as a function of dTTP concentration and fit with Equation 4 to yield kp (0.0015 ± 0.0001 s−1) and Kd (0.39 ± 0.08 mM).
