Table 1.

Pre-Steady-State Kinetic Parameters of Dpo3

dNTP	kp (s−1)1	Kd (mM)1	kp/Kd (μM−1 s−1)	Misincorporation Frequency2
Template A
dATP	0.0010 ± 0.0002	0.23 ± 0.02	4.5 × 10−6	6.7 × 10−3
dCTP	0.0016 ± 0.0002	2.0 ± 0.6	7.9 × 10−7	1.2 × 10−3
dGTP	0.0017 ± 0.0004	1.7 ± 1.1	9.6 × 10−7	1.5 × 10−3
dTTP	0.038 ± 0.002	0.057 ± 0.008	6.6 × 10−4
Template C
dATP	0.00065 ± 0.00005	0.74 ± 0.20	8.9 × 10−7	3.0 × 10−4
dCTP3	-	-	-	-
dGTP	0.069 ± 0.005	0.024 ± 0.007	2.9 × 10−3
dTTP	0.0013 ± 0.0001	0.43 ± 0.16	3.1 × 10−6	1.1 × 10−3
Template G
dATP	0.0025 ± 0.0005	1.9 ± 0.9	1.3 × 10−6	1.9 × 10−3
dCTP	0.045 ± 0.008	0.061 ± 0.025	7.4 × 10−4
dGTP4	0.0011 ± 0.0001	0.056 ± 0.017	2.0 × 10−5	2.8 × 10−2
dTTP	0.0016 ± 0.0001	0.30 ± 0.08	5.3 × 10−6	7.6 × 10−3
Template (T)G
dGTP	0.0021 ± 0.0002	0.69 ± 0.20	3.1 × 10−6	4.4 × 10−3
Template T
dATP	0.12 ± 0.01	0.018 ± 0.006	6.8 × 10−3
dCTP	0.0009 ± 0.0001	0.79 ± 0.34	1.1 × 10−6	1.6 × 10−4
dGTP5	0.0025 ± 0.0001	0.41 ± 0.03	6.0 × 10−6	8.7 × 10−4
dTTP	0.0015 ± 0.0001	0.39 ± 0.08	3.7 × 10−6	5.4 × 10−4

¹Calculated from the fit to Equation 4 of the second order plot.

²Calculated as (k_p/K_d)_incorrect/(k_p/K_d)_correct.

³Was not incorporated appreciably above background and unable to quantify.

⁴Inhibition at [dGTP] > 1mM.

⁵Inhibition at [dGTP] > 2 mM.