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. Author manuscript; available in PMC: 2013 Apr 12.
Published in final edited form as: DNA Repair (Amst). 2012 Feb 15;11(4):381–390. doi: 10.1016/j.dnarep.2012.01.004

Fig. 1.

Fig. 1

C127 cell lines inducibly express exogenous Pol β. Representative Western blots showing expression of WT Clone 1, D160N Clone 16. Exogenous Pol β is HA-tagged and runs higher than the untagged endogenous form. Blots were quantified using ImageQuant Software. Under inducing conditions (NT), WT clone 1 expresses at a ratio (exogenous:endogenous) of 0.97:1. D160N Clone 16 expresses at a ratio of 0.5:1. Adding Tet back to the media shuts off exogenous expression in D160N Clone 16 (shown for passage 22), as the ratio is reduced to 0.1:1.