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. Author manuscript; available in PMC: 2013 Apr 12.
Published in final edited form as: DNA Repair (Amst). 2012 Feb 15;11(4):381–390. doi: 10.1016/j.dnarep.2012.01.004

Fig. 5.

Fig. 5

D160N maintains interactions with BER binding partners. A pull-down experiment was performed using WCE from Pol β-deficient MEFs and his-tagged purified Pol β enzyme as described in Section 2. (A) WT and D160N interact with XRCC1 (90 kDa). Lane 1, 25% input; Lane 2, WT with nickel beads alone; Lane 3, D160N with nickel beads alone; Lane 4, WCE with nickel beads alone; Lane 5, WT and WCE pull-down; Lane 6, D160N and WCE pulldown. (B) WT and D160N interact with LigaseIIIα (103 kDa). Lane 1, 25% input; Lane 2, WT with nickel beads alone; Lane 3, D160N with nickel beads alone; Lane 4, WCE with nickel beads alone; Lane 5, WT and WCE pulldown; Lane 6, D160N and WCE pulldown.