Table 3.
Type of error | Frequency × 10−4 (no. events)
|
Ratio D160N/β-WT | |
---|---|---|---|
D160N | β-WT | ||
Pol EFest (HSV-tk coding region) | 310 (90) | 25 (68) | 12 |
Base substitutions (independent) | 55 (16) | 2 (6) | 28 |
Frameshifts (independent) | 138 (40) | 16 (43) | 9 |
Tandem errors | 117 (34) | 7 (19) | 17 |
Simple | 31 (9) | 6 (17) | 5 |
Complex | 86 (25) | 0.7 (2) | 123 |
The HSV-tk coding region Pol EF from Table 2 was averaged for both substrates, and the average used to calculate frequencies of specific error classes. Base substitutions and frameshifts are considered independent if they occur greater than 15 nucleotides apart. Tandem simple mutations refer to two single mutations that occur within 15 bases. Tandem complex mutations describe >2 mutations that occur within 15 bases.