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. Author manuscript; available in PMC: 2013 Apr 12.
Published in final edited form as: J Cell Physiol. 2008 May;215(2):287–291. doi: 10.1002/jcp.21380

Figure 2. Immunofluorescence analysis of MAGE-A antigens expression in NA and A WM3130 melanoma cells.

Figure 2

NA and A WM3130 cells were fixed in 4% paraformaldehyde, blocked in 1:50 fold dilution of goat serum in 1× PBS/0.1% Triton-X 100, and incubated with the anti-MAGE-A mAb clone 6C1 or with mouse IgG (mIgG), as a control. After washing, cells were incubated with goat anti-mouse Alexafluor 488 secondary antibody, and nuclei were stained with Hoechst 03323. Photographs were taken at 20× magnification.