Figure 9. Effects of SEA0400 on the augmentation of Glu-evoked neuronal Ca²⁺ signals by ouabain.

A, effects of 300 μm SEA0400 on the Ca²⁺ transients evoked by 3 μm Glu. SEA0400 was dissolved in DMSO (final concentration of 0.367%) which, alone, had no effect on the Ca²⁺ transients (data not shown, but see Fig. 3B). Aa, data from a representative neurone illustrate the protocol. Ab, summarized data from 11 neurones show that SEA0400 did not significantly affect the Glu-evoked Ca²⁺ signals. B, effect of 3 nm ouabain ± 300 μm SEA0400 on 3 μm Glu-evoked Ca²⁺ transients. Ba, data from a representative neurone; inset shows the neurone (arrow) sitting on a layer of astrocytes. Bb, summary of data from 11 neurones. NS, not significantly different; ***P < 0.001, for the indicated pairs (ANOVA).