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. 2013 Jan 14;591(Pt 7):1951–1966. doi: 10.1113/jphysiol.2012.246983

Figure 4. Enhanced phosphorylation of CREB (pCREB) induced by cocaine treatment in hypocretin neurons.

Figure 4

Dual immunostaining of pCREB and hypocretin (Hcrt) was performed in control (n= 3) and cocaine-treated mice (n= 3) after a 3-day treatment. AC, images show that only hypocretin-immunopositive neurons (indicated by arrowheads) are present in the perifornical/LH area in saline-treated mice. DF, images show that hypocretin and pCREB-immunopositive neurons (indicated by arrows) are present in the LH area in cocaine-treated mice. Scale bar: 15 μm. G, pooled data from all examined sections from saline- (n= 24 sections) and cocaine-treated (n= 21 sections) mice indicate a significant increase in pCREB expression in hypocretin neurons following a 3-day cocaine treatment. *P < 0.001, t test.