Table 1.

Nucleotide sequences of primers used in single-cell RT-PCR experiments

Target	Accession no.	Forward	Reverse	Size (bp)
T1R1	NM_031867	AGGGGGACCCTCTAGGTTAT	ATGATGACCAGTTGGAAGGA	651
		GGTTCTGCCTCACTGTCTCC	GCAGCAGCAATAGCGTGTTA	339
T1R2	NM_031873	GCACCAAGCAAATCGTCTAT	CCAGAAGATGAGCAGAATGG	536
		CTCAGGGAGATCTGGCATGT	GTGGAGGCCTATGGGTTTTT	273
T1R3	NM_031872	TGCCTGAATTTTCCCATTAT	AGGACACTGAGGCAGAAGAG	889
		CTACCCTGGCAGCTCCTGGA	CAGGTGAAGTCATCTGGATGCTT	343
TRPM5	NM_020277	TTCCTGTTCATTGTGGGAGT	GACTCCTGCAACCACAGTTC	971
		CTGATCGCCATGTTCAGCTA	ACTCTGTGTGCCGTTTTCCT	335
PLCβ2	NM_177568	TTTGGAGAGACCTTGCTCAC	CCATTGTCTGGAAATTGAGG	628
		GGTGGAAGAGGAGGAAGAGG	CTGACATCCAGCATTCCAGA	352
GLAST	NM_148938	GGTAAAATCGTGCAGGTCAC	CCACACCATTGTTCTCTTCC	673
		ACATGTTCCCTCCCAATCTG	CAAGAAGAGGATGCCCAGAG	362
Gustducin	NM_001081143	ACGAGATGCAAGAACTGTGA	TATCTGTCACGGCATCAAAC	941
		TGCTTTGAAGGAGTGACGTG	GTAGCGCAGGTCATGTGAGA	341
SNAP25	NM_011428	AAGGGATGGACCAAATCAAT	CAATGGGGGTGACTACTCTG	601
		AAAAAGCCTGGGGCAATAAT	AGCATCTTTGTTGCACGTTG	304
GAD67	NM_008077	CATTCTGGTCAAGGAAAAGG	CAGCTCTAGCAGGGAGGTAG	986
		AGATAGCCCTGAGCGACGAG	ATGGCCGATGATTCTGGTTC	240
β-Actin	NM_007393	CCTGAAGTACCCCATTGAAC	GTAACAGTCCGCCTAGAAGC	943
		GGTTCCGATGCCCTGAGGCTC	ACTTGCGGTGCACGATGGAGG	370

Upper sequences are outside primers and lower sequences inside primers. Abbreviations: GAD67, glutamate decarboxylase 67; GLAST, glutamate/aspartate transporter; PLCβ2, phospholipase C β2; SNAP25, synaptosomal-associated protein 25; T1R1 (T1R2 or T1R3), taste receptor family 1 member 1 (2 or 3); and TRPM5, transient receptor potential cation channel subfamily M member 5.