Figure 2.

EBI3 deficiency but not p28 deficiency protects mice from colitis. (A) Targeting strategy for generation of IL-27p28 – deficient mice. Mice were genotyped using PCR with tail DNA using a common primer upstream of exon 2 and a genotype-specific reverse primer within the p28 gene (wild-type allele) or the LacZ region (targeted allele), respectively (right). (B) Serum IL-27p28 protein levels of wild-type or targeted mice 24 hours after intraperitoneal injection of 10 mg/kg lipopolysaccharide (LPS) (left) or isolated bone marrow derived dendritic cells (BMDC) (right). IL-27p28 levels were determined by enzyme-linked immunosorbent assay. One representative experiment of 2 is shown. N = 4 –5/group. (C) Survival analysis of EBI3^−/− or IL-27p28^−/− mice or controls with conditional ablation of STAT3 in myeloid cells (N = 7–10/group). (D) Weight analysis of EBI3- and p28-deficient RAG^−/− mice adoptively transferred with 0.5 × 10⁶ CD4⁺ CD25⁻ T cells (N = 9 –13/group). (E) Survival analysis of EBI3^−/− or IL-27p28^−/− mice or controls after oral treatment with 3% DSS at indicated time points (n = 8 –12/group). *P < .05; **P < .01; ***P < .001.