Figure 4.
Attenuated pro-inflammatory responses in IL-10−/− mice monoassociated with NC101 compared to NC101ΔibpAB are not proportional to differences in luminal bacterial concentrations at early time points. Luminal bacterial concentrations in the cecum (A) or mid-colon (B) from IL-10−/− mice monoassociated with NC101 or NC101ΔibpAB. (C) Spontaneous IL-12/23p40 secretion in proximal colon explant cultures. (D) IFN-γ secretion by unseparated MLN cells stimulated ex vivo with NC101 lysate. (E) Blinded composite histological inflammation scores of 4 colonic segments. Data are means +/− SEM, n=5–6 mice/group, *p<0.05 relative to NC101-monoassociated mice.