Fig. 4.

CD8+ γ-H2AX+ T lymphocytes are more frequent within the liver of HCV-infected subjects. (A) Example plots of CD8 and γ-H2AX expression on peripheral (left panel) and intrahepatic (right panel) lymphocytes from an HCV-RNA+ subject. (B) Expression of γ-H2AX on circulating and intrahepatic CD8+ T lymphocytes from 6 HCV infected subjects. Analysis by Wilcoxon signed rank test. (C) Co-expression of CD27 and γ-H2AX on intrahepatic CD8+ T lymphocytes. (D) Response of hepatic CD8+ T lymphocytes to in vitro IFN-α through STAT1 phosphorylation. Example dot-plots of hepatic CD8+ T lymphocytes incubated with 10 IU/ml (left panel) or 1000 IU/ml IFN-α2b (right panel).