Figure 6. Apoptosis assay (Annexin V/PI assay).

A, Apoptosis assay was performed after T24 cells were cultured in RPMI-1640 containing hWJMSC-MVs (0, 100 or 200 µg/ml protein) for 24, 48 and 72h, respectively. B, Apoptosis index analysis. Annexin V-FITC positive cells were expressed as mean±SD from three different replicates. The “*” and “#” indicate statistical significance at P<0.05 compared to respective controls within the group and between the groups, respectively.