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. 2013 Apr 12;8(4):e61366. doi: 10.1371/journal.pone.0061366

Figure 7. hWJMSC-MVs inhibited the phosphorylation of Akt and activated Caspase 3, p53 and p21.

Figure 7

T24 cells were incubated with hWJMSC-MVs for indicated time periods, Akt, p-Akt, cleaved Caspase 3 expression were examined by Western blot analysis. A, After being exposed to hWJMSC-MVs for 24, 48 and 72h, T24 cell growth was significantly hindered by down-regulating the phosphorylation of Akt and up-regulating cleaved Caspase 3. B, Compared with T24 cells exposed to hWJMSC-MVs for only 24h, the increase of exposure time (48 or 72h) showed more effective inhibition on phosphorylation of Akt (p<0.01) (a). Conversely, expression of cleaved Caspase 3 was significantly up-regulated when T24 cells were exposed to hWJMSC-MVs for 72h than for 24 or 48h (p<0.01) (b). C, D, After being treated with hWJMSC-MVs for 24 and 48h, p-p53 and p21 were dramatically up-regulated in comparison with the controls (0h). Independent experiments were repeated up to three times with similar results. The “*” and “#” indicate statistical significance at P<0.05 in comparison with respective controls within the group and between the groups, respectively.