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. 2013 Apr 12;8(4):e61915. doi: 10.1371/journal.pone.0061915

Figure 2. Identification of potent migration inhibiting genes.

Figure 2

Seven genes from the screening hits were confirmed to affect U87 cell migration. (A) Column 1, a representative area showing the migrated cells attached to the lower membrane surface of the Matrigel invasion chamber. Cells were either mock transduced or transduced with shRNAs targeting the indicated genes. Columns 2 to 4, images showing cell migration in a wound healing experiment at indicated time points. Scale bar, 100 µm. (B) Quantification of the cell migration in the Matrigel invasion chamber experiments. n = 3. *, P<0.05. (C) Quantification of the cell migration in the wound healing experiments. n = 3. *, P<0.05. (D) Confirmation of the protein knockdown by western blotting.