Skip to main content
. 2013 Apr 12;8(4):e61915. doi: 10.1371/journal.pone.0061915

Figure 4. Validation of the gene effects with other GBM cells and secondary shRNAs.

Figure 4

(A) The effect of the shRNAs on GBM cell lines A172, LN-229 and primary GBM tumor cells. Experiments were carried out using Matrigel invasion chamber. *, P<0.05, n = 3. (B) Protein expression change after the treatment of a secondary shRNA sequence targeting genes HCFC1, FLNA and KHSRP. (C) The effect of the secondary shRNAs on U87 cell migration. Experiments were carried out using Matrigel invasion chamber. *, P<0.05, n = 3.