Table 1. Genes identified in the RNAi screening.
Gene | Target sequence | Colony frequency | Inhibition ranking |
FIGNL1 | CCAGGAAACAGATAGTAAT | 68 (22.7%) | 44.6±8.8% |
SENP8 | CTGGCTCAATGACCATATT | 39 (13.0%) | 55.7±10.1% |
LCTL | GAAACTTGCTCTATCAACA | 33 (11.0%) | 51.8±13.4% |
VAV1 | GGCAGAAATACATCTACTA | 32 (10.7%) | N/A |
HCFC1 | CAACCACCATCGGAAATAA | 20 (6.7%) | 86.6±7.5% |
GOLGA6L5 | AGCTAAACATCACCATCAT | 16 (5.3%) | N/A |
B3GAT2 | AAATAACTGCACTAAGGT | 12 (4.0%) | 87.9±7.3% |
FLNA | CCTACTTTGAGATCTTTA | 12 (4.0%) | N/A |
KHSRP | CGAGAAGATTGCTCATATA | 11 (3.7%) | 95.5±1.0% |
DLK1 | CACATGCTGCGGAAGAAGA | 8 (2.7%) | 77.5±9.3% |
PROKR1 | CCTGGTCCGCTACAAGAAA | 6 (2.0%) | 94.3±4.1% |
TERF1 | GTAATGATGTTGAAATGGAA | 6 (2.0%) | N/A |
LRRIQ3 | CTCACTTTAACTTACCAAA | 5 (1.7%) | 85.3±12.8% |
TWF1 | CAACTTGTGATTGGATCAT | 4 (1.3%) | 82.9±8.0% |
NOB1 | CTCCTGTGCATTTAATTAA | 3 (1.0%) | 90.3±8.8% |
ERCC2 | CTCACCGACTGCTTCCTGA | 3 (1.0%) | N/A |
RIPK1 | ACCAACAGATGAATCTATA | 2 (0.7%) | 15.8±15.9% |
HEPHL1 | CCCAACAGGATAGGCAGTA | 2 (0.7%) | 41.1±14.7% |
SMAD1 | CTATTTCATCTGTATCTT | 2 (0.7%) | 85.3±11.1% |
XPO4 | CAGCGATTCTTAAGAGTGA | 2 (0.7%) | 52.9±17.3% |
BUB1 | CAGGAAAGGTCCGAGGTTA | 2 (0.7%) | 27.3±7.9% |
AMMECR1 | CTCCTTCCTTCCACATTTA | 2 (0.7%) | 80.9±6.5% |
VPS18 | CATTGTACGTGCTAAATGA | 2 (0.7%) | 33.6±11.4% |
DUSP12 | GTCGAAGTGTGGCCATAAT | 2 (0.7%) | 88.2±5.7% |
CCNC | CTCCTTTCATGATAGCTTT | 2 (0.7%) | 68.5±10.0% |