Figure 2. LPL activity and fatty acid uptake are reduced in WAT of SMS1-KO mice.

(A) Shown are triglyceride levels in blood plasma of 14-week-old mice (WT, n = 3; KO, n = 3). (B) LPL activity was assayed in WAT and liver of 20-week-old mice (WT, n = 6; KO, n = 5). (C) [³H]palmitic acid was intraperitoneally injected to 4 h-starved mice. After 0.5 h (WT, n = 8; KO, n = 5) or 48 h (WT, n = 7; KO, n = 3), tissues were isolated and radioactivity in tissues was measured. (D) Assessment of [³H]palmitic acid uptake into MEFs isolated from wild-type (WT, n = 30) and SMS1-deficient (KO, n = 30) embryos.