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. 2013 Apr 12;8(4):e60718. doi: 10.1371/journal.pone.0060718

Figure 9. Cholesterol does not cause DSBs in hTERT-HM1 cells.

Figure 9

(A) hTERT-HME1 cells were exposed to 4 µg/ml of cholesterol for 48 hr and immunostained for a double-strand breaks (DSBs) marker, the p53 binding protein 53 BP1. Immunofluorescent 53 BP1 (green) foci in nuclei (DAPI, blue) of control and cholesterol-treated cells were counted. (B) There was no statistically significant (p = 0.06) increase in DSBs events (≥3 foci per nucleus) in cholesterol-treated compared to untreated cells. The nuclei containing ≥4 foci were extremely rare.