Figure 4. Preincubation with forskolin prevent histamine-induced increases in F-actin.

PCA rings were treated the same way as described in Figure 3. Panel A: Treated tissues were homogonized and F- and G-actin were separated by centrifugation, F-actin was converted to G-actin, and measured by western blot, and quantitated compared to the standard actin loaded on the same gel. Panel B: F-actin concentration was calculated and cumulative data is plotted n = 7, p<0.05. *significance between Histamine and all other groups.