Figure 6. Preincubation with forskolin reduces phosphorylation of cofilin in PCA.

PCA rings were treated with the same conditions as described in Figure 3. Proteins were extracted and separated on SDS polyacrylamide gels and p-cofilin was detected by western blotting. Panel A: Representative western blot of p-cofilin and np-cofilin. Panel B: Cumulative data representing the relative amounts of p-cofilin over the total cofilin and then normalized to the basal. Quantitative values were obtained when the phosphorylated and non-phosphorylated bands were quantitated densitometrically. n = 4, p<0.05. *significant compared to all other conditions.