Figure 3. E. coli expressed/refolded PF11_0521 DBL2 domain binds ICAM-1 but has ∼3 times lower specific activity than COS-7 expressed domain.

Amount of protein on beads was measured by reactivity of rat plasma IgG raised against E. coli-expressed full-length DBL2 domain (in arbitrary units, AU). Error bars represent Standard Deviations. Dilution used (1∶32000) is in the linear range of titration curve for these antibodies. Specific activity = AU(ICAM-1 binding)/AU(IgG reactivity).