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A. Antibodies against “short” (top) and “3-helix” (bottom) domains do not inhibit ICAM-1 binding to COS-expressed full-length domain. Pre-Im, pre-immune plasma as negative control. Error bars represent Standard Deviations. B. Antibodies against full-length domain can completely inhibit ICAM-1 binding to COS-expressed full-length domain. Mouse antibodies were titrated down to 1∶1000 dilution.
