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. 2013 Apr 12;8(4):e61281. doi: 10.1371/journal.pone.0061281

Figure 5. M and NP localization below the plasma membrane of infected cells.

Figure 5

A549 cells were infected with the indicated viruses for 16 h at 34°C. Cells were processed for IF using anti-SeV NP mAb or M rabbit serum. Top 3 panels represent localization of M and NP. Merged images are deconvoluted z-stack images of the xy-plane created using an Olympus FV1000 confocal microcope. Bottom 3 panels show plasma membrane, M and NP and are z-stack reconstructions of the ROIs indicated in the whole cell images by white rectangles in the merged images.