Figure 4. CD109 Protein expression over the days of OCG.

Western blot analysis showing that CD109 protein expression is increased in the critical days of osteoclastogenesis in H10 cells, RAW264.7 cells and BMMs (n = 3, P<0.05). Band intensity of CD109 expression was normalized with β-actin. Band intensity of C8 cells was used as the baseline for 4A. Day 1 for 4B, and Day 0 for 4C.