Figure 2. Quantification of SMP30, Ferritin (Ftl), Hepcidin (Hamp), Creb3l3, Nuclear factor erythroid derived 2 like 2 (Nfe2l2) and interleukin-1 beta (Il1b) mRNA from the livers of adult rats treated with control and restricted-iron (ARI) or enriched-iron (AEI) diets and of young rats (Gene/b-actin mRNA ratio - fold change).

Average and standard deviation: n = 7 (CT); n = 5 (ARI); n = 8 (AEI); n = 6 (young). Difference between control group and test groups: *p≤0.05; **p≤0.01; ***p≤0.001 (Student's t-test). RT-PCR analyses were normalized against the mRNA expression of the housekeeping gene (β-actin gene) in the same tissue sample and then expressed as the “test/control” ratios.