Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Apr 12;8(4):e60869. doi: 10.1371/journal.pone.0060869

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Marelja et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Subcellular EGFP assembly of different split-EGFP fusion proteins was analyzed in HeLa cells by confocal fluorescent microscopy. The following fusion proteins were expressed after cotransfection (assembly of EGFP^1–157 and EGFP^158–238 resulted in a green pseudocolor): A, ISD11-EGFP^1–157 and NFS1-EGFP^158–238; B, MOCS3-EGFP^1–157 and NFS1-EGFP^158–238; C, MOCS3-EGFP^1–157 and NFS1Δ1-55-EGFP^158–238; D, MOCS3-EGFP^1–157 and MOCS3-EGFP^158–238; E, NFS1-EGFP^1–157 and NFS1-EGFP^158–238; F, ISD11-EGFP^1–157 and NFS1Δ1-55-EGFP^158–238. Mitochondria of HeLa cells were visualized with MitoTracker® DeepRed (red) or the nuclei were visualized with DAPI stain (magenta). Merged pictures are shown right (either resulting in a yellow or white color). Scale bars, 20 µm; scale bars in the insets, 2 µm.