Fig. 2.

E2f3a and E2f3b induce Igf2 mRNA expression in late juvenile but not in fetal hepatocytes. E2f3a and E2f33b expression vectors were transiently transfected into primary hepatocytes isolated from mice at 5 wks of age (Upper) or at E15 (Lower), and Igf2 transcript variants were measured 2 d later by real-time PCR. Variant 1 expression showed >20-fold induction by E2f3a and E2f3b in late juvenile hepatocytes, but not in fetal hepatocytes. Data are shown as mean ± SEM; v, variant; *P < 0.05 vs. control, ANOVA.