Fig. 5.
TDAV viral load and liver enzyme function in animals experimentally inoculated with antitoxin 2. (A) qRT-PCR quantification of TDAV RNA. Cycles to threshold (Ct) values from the qRT-PCR assay [average values and SD (n = 4) with primers EVT-146/147] are plotted on an inverted y axis; a lower Ct value represents a greater viral load. (B–E) Biochemical tests for AST, GGT, SDH, and GLDH. Samples were taken at time 0 and weekly for 10–14 wk after inoculation with 500 mL of the same lot of antitoxin 2 linked to the serum hepatitis outbreak. One horse became symptomatic (horse C1, blue), whereas the other three did not (horse C2, green; horse C3, orange; and horse C4, black).